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Uses and synthesis of Horseradish Peroxidase powderHorseradish Peroxidase powder is a glycoprotein combined with iron porphyrin. The enzyme is composed of multiple isoenzymes. Horseradish Peroxidase is a kind of oxidoreductase produced by microorganism or plant, which can catalyze many reactions. Horseradish Peroxidase is an enzyme that catalyzes substrate oxidation with hydrogen peroxide as electron acceptor. It mainly exists in the peroxisome of the carrier. With iron porphyrin as the cogroup, it can catalyze hydrogen peroxide and oxidize phenols, amines and hydrocarbon oxidation products. Horseradish Peroxidase powder can eliminate the toxicity of hydrogen peroxide and phenols, amines, aldehydes and benzene.
Applications/Functions of Horseradish Peroxidase powder
Horseradish Peroxidase powder is used in biochemical research, which is a major enzyme used in enzyme labeling method. It is used in determining the content of glucose and galactose in biological fluid and used as a diagnostic enzyme in medicine. It is used to make scavengers, which can degrade and eliminate phenols, amines, formaldehyde, toluene and other pollutants by supporting enzymes.
Physicochemical Property of Horseradish Peroxidase powder
Brown crystalline substance or freeze-dried powder, soluble in water, commercial ammonium sulfate suspension, pI7.2, optimal pH 7.0. Stability: Freeze-dried powder and 2.8mol/L ammonium sulfate suspension can be stable for one year at 4℃.
Production methodprocess of Horseradish Peroxidase powder
Horseradish Peroxidase was extracted from horseradish root with water and purified by ammonium sulfate for the first time, and purified by calcium phosphate gel. Then, Horseradish peroxidase was synthesized by ethanol and ammonium sulfate for the second time.
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